Fig. 2

Cellular proteome profiling of K. pneumoniae cultured in zinc-limited and -replete media. a Venn diagram depicting total unique proteins identified in limited (red, 15) and replete (blue, 99) conditions. b Principal component analysis. Variance amongst biological replicates of cells grown in limited (red, top) and replete (blue, bottom) conditions was separated based on component 1 (40.7%) and component 2 (30.4%). c 1D-annotation enrichment. UniProt keywords enriched in the replete condition relative to the limited. Statistical significance confirmed by Student’s t-test (p-value ≤ 0.05, FDR = 0.05, score < − 0.5, < 0.5). d Volcano plot depicting proteins present in cellular proteome. Proteins in blue (right) are significantly abundant under replete conditions. Proteins in red (left) are significantly abundant under limited conditions. Statistical analysis was performed using a Student’s t-test (p-value ≤ 0.05; FDR = 0.05; S0 = 1). Experiment performed in biological quadruplicate and technical duplicate