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Table 1 Steps of purification and percent yield of enterocin 12a purified from E. faecium 12a

From: Anticancer and antimicrobial potential of enterocin 12a from Enterococcus faecium

Purification step Total volume (ml) Activity
(AU/ml)
Total activitya Total Protein
(mg)
Specific
activityb
Fold-Increase in the specific activity Percent Yield
CS 1000 68,266 6.8× 107 625 108,800 1 100
Ammonium sulphate ppt. 200 136,528 2.7×107 215 125,581 1.15 39.7
Cation-exchange fraction 30 273,056 8.1× 106 33 245,455 2.26 11.9
Reverse-phase chromatography 2 546,112 1.1×106 1 1,100,000 10.11 1.6
  1. aTotal activity= Activity × Total volume of sample
  2. bSpecific activity = Total activity/ Total protein