Table 1 Steps of purification and percent yield of enterocin 12a purified from E. faecium 12a
From: Anticancer and antimicrobial potential of enterocin 12a from Enterococcus faecium
Purification step | Total volume (ml) | Activity (AU/ml) | Total activitya | Total Protein (mg) | Specific activityb | Fold-Increase in the specific activity | Percent Yield |
|---|---|---|---|---|---|---|---|
CS | 1000 | 68,266 | 6.8× 107 | 625 | 108,800 | 1 | 100 |
Ammonium sulphate ppt. | 200 | 136,528 | 2.7×107 | 215 | 125,581 | 1.15 | 39.7 |
Cation-exchange fraction | 30 | 273,056 | 8.1× 106 | 33 | 245,455 | 2.26 | 11.9 |
Reverse-phase chromatography | 2 | 546,112 | 1.1×106 | 1 | 1,100,000 | 10.11 | 1.6 |