Fig. 3

Histograms depicting PI fluorescence of S. enterica cells a) untreated control b) enterocin 12a-treatment for 15 min c) enterocin 12a-treatment for 30 min. The cells of S. enterica were treated with 5 μg/ml of enterocin for 15 and 30 min. The cells were washed by centrifugation and stained with PI. The fluorescence of the PI-stained cells was determined by flow cytometer. Data are represented as histograms with counted bacterial events displayed on y-axis, and increase in fluorescence on the x-axis