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Fig. 2 | BMC Microbiology

Fig. 2

From: Anticancer and antimicrobial potential of enterocin 12a from Enterococcus faecium

Fig. 2

(a) U-HPLC chromatogram of the purified enterocin 12a (b) SDS-PAGE of purified enterocin 12a and gel overlay with S. enterica. Lane 1: molecular weight marker. Lane 2: partially-purified enterocin 12a after cation exchange chromatography. Lane 3: purified enterocin 12a after RP-HPLC. Lane 4: Zone of inhibition against S. enterica observed in the agar gel overlay assay corresponding to the position of the band on the SDS-PAGE of enterocin 12a (c) Peptide mass fingerprinting (PMF) analysis of enterocin 12a. The PMF followed by MASCOT search in NCBIprot shows the matched amino acid residues (in red bold) of the seven peptide fragments with the 4Fe-4S domain-containing protein of E. faecalis R712

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