Table 2 Strains and plasmids
Organism | Purpose | Ref. |
DH5α | Expression host of lux reporter assay | Promega |
TOP 10 | Transformation and propagation of plasmids | Invitrogen |
D. circinale AWQC131C | Organism harbouring native sxt cluster | [34] |
Synechocystis sp. PCC6803 | Expression host of lux reporter assay | Â |
Synechocystis SXL7 | ΔphbC:: PsxtD-luxCDABE - SpecR | This study |
Synechocystis SXL8 | ΔphbC:: PsxtP-luxCDABE - SpecR | This study |
Synechocystis SXL9 | ΔphbC:: PsxtPER1-luxCDABE - SpecR | This study |
Synechocystis SXL10 | ΔphbC:: Porf24-luxCDABE - SpecR | This study |
Synechocystis SXL11 | ΔphbC:: luxCDABE - SpecR | This study |
Plasmids | Purpose | Ref. |
pCR™2.1 TOPO | Cloning and sequencing of 5′ RACE TSS products | Invitrogen |
pGEM T-Easy | Cloning and sequencing of 5′ RACE TSS products | Promega |
pET28b | Cloning and expression of sxt promoters | Novagen |
pSYN_6.3 | Cloning and transformation of sxt promoters in Synehcocystis sp. PCC 6803 | Unpublished |
pLUX NS II | luxCDABE | [59] |
pSXL1 | pET28b::PsxtD-luxCDABE | This study |
pSXL2 | pET28b::PsxtP-luxCDABE | This study |
pSXL3 | pET28b::PsxtPER1-luxCDABE | This study |
pSXL4 | pET28b::Porf24-luxCDABE | This study |
pSXL5 | pET28b::PsxtPER2-luxCDABE | This study |
pSXL6 | pET28-sxtO-luxCDABE | This study |
pET28-lux | pET28-luxCDABE | This study |
pSXL7 | pSYN_6.3::PsxtD- luxCDABE | This study |
pSXL8 | pSYN_6.3::PsxtP- luxCDABE | This study |
pSXL9 | pSYN_6.3::PsxtPER1- luxCDABE | This study |
pSXL10 | pSYN_6.3::Porf24- luxCDABE | This study |
pSXL11 | pSYN_6.3::luxCDABE | This study |