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Fig. 4 | BMC Microbiology

Fig. 4

From: Identification of promoter elements in the Dolichospermum circinale AWQC131C saxitoxin gene cluster and the experimental analysis of their use for heterologous expression

Fig. 4

Heterologous expression of luciferase from sxt promoters in Synechocystis sp. PCC 6803. a Luciferase expression from the four main sxt promoters in Synechocystis sp. PCC 6803. The highest RLU/OD for the Synechocystis sp.PCC 6803 strains expressing luxCDABE. (*) Denotes statistically significant differences (p < 0.05) between the Synechocystis sp. PCC 6803 strains and the Synechcocystis sp. PCC 6803-lx control in unpaired t-tests. b Normalised luciferase expression over time in Synechocystis PCC6803. RLU/OD730 over 400 h of growth. PsxtD normalised expression was low. PsxtP and Porf24 had consistent levels of luciferase expression. PsxtPER showed high initial expression that declined over growth to expression reach levels similar to PsxtP and Porf24

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BMC Microbiology

ISSN: 1471-2180

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