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Fig. 3 | BMC Microbiology

Fig. 3

From: Identification of promoter elements in the Dolichospermum circinale AWQC131C saxitoxin gene cluster and the experimental analysis of their use for heterologous expression

Fig. 3

Heterologous expression from of luciferase from sxt promoters in E. coli DH5α. a Luciferase expression was normalised to the optical density, OD600. The activity of five promoters was tested: PsxtD, PsxtP, PsxtPER1, Porf24, and PsxtPER2. An intergenic sxtO sequence was used as the non-promoter control. The highest RLU/OD for the E. coli DH5α strains expressing luxCDABE. (*) Denotes statistically significant differences (p < 0.0001) between the E. coli DH5α strains and the pET28-lux control in unpaired t-tests. b Expression relative to the sxtO-lux control. The mean normalised luciferase expression for each promoter was divided by the mean normalised expression of sxtO. The three strongest promoters (PsxtD, PsxtP, PsxtPER) exhibit between 1000 and 9500 fold higher levels of luciferase expression over the sxtO control. The Porf24 and PsxtPER2 promoters had only a 12 and 27-fold increase in expression, respectively

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BMC Microbiology

ISSN: 1471-2180

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