Table 3 Bacterial strains and plasmids used in the present study
Bacterial strain or plasmid | Description | Source |
|---|---|---|
Bacterial strains | ||
E.faecalis OG1RF | Rifampin- and fusidic acid-resistant derivative of human oral cavity isolate | ATCC |
 △clpP mutant | clpP deletion mutant obtained using OG1RF as the parent strain | This study |
 △clpP/pIB166::clpP | clpP deletion mutant complemented with plasmid pIB166 harboring the clpP gene | This study |
 △clpP/ pIB 166 | clpP deletion mutant complemented with plasmid pIB166 | This study |
E.faecalis ATCC29212 | Standard strain for the MIC detection | ATCC |
Plasmids | ||
 pJRS233 | Temperature-sensitive E. coli (Erm750)-Enterococcus (Erm25) shuttle vector | Michael G. Caparon, Washington University |
 pJRS233-△clpP | Temperature-sensitive plasmid for generation of in-frame deletion of clpP | This study |
 pIB166 | E. coli (Cm20)-Streptococcus (Cm20) shuttle vector | Jingren Zhang, Tsinghua University |
 pIB166::clpP | Used for complementation of clpP deletion | This study |