Fig. 4

Phenotypic characterization of mutation in six motility-related (pili, flagellar and chemotaxis) genes. a Examination of swimming and swarming motility. Left side, strains were inoculated onto ‘swarm’ plate (NY medium containing 2% glucose and 0.6% agar) then incubated at 28 °C for 3 days. Right side, strains were stabbed into ‘swim’ plate (0.03% Bacto peptone, 0.03% yeast extract and 0.28% agar) then incubated at 28 °C for 3 days. b The diameter of the strains on swimming and swarming plates. c Chemotaxis response assay. The strains were diluted and performed simplified capillary assays for 8 chemoattractant, and counted bacterial CFUs after 3 days of incubation at 28 °C. Relative chemotaxis fraction was calculated CFU in test capillary vs CFU in control buffer capillary. All datas were representative of three independent experiments. Significance was tested by Student’s t test (* and ** represent significance at P < 0.05 and 0.01, respectively)