Fig. 3

Epidemic ribotype infected mice had significantly more fecal-associated Toxin A and B than mice infected with non-epidemic ribotype isolates of C. difficile. For each isolate, groups (n = 20) were housed 5 to a cage and inoculated with approximately 1 × 10⁶ C. difficile spores. Fecal pellets were then collected, weighed, and processed to measure Toxin A and B concentrations via ELISA. a Mean Toxin A titers per gram of feces that was collected from epidemic or non-epidemic ribotype infected mice on days 0 to 12 of the studies. b Mean Toxin B titers per gram of feces that was collected from epidemic or non-epidemic ribotype infected mice on days 0 to 12 of the studies. c Normalized mean Toxin A titers per CFU that was collected from epidemic or non-epidemic ribotype infected mice on days 0 to 12 of the studies. d Normalized mean Toxin B titers per CFU that was collected from epidemic or non-epidemic ribotype infected mice on days 0 to 12 of the studies. These data represent the average of four independent groups, and error bars indicate the standard errors of the means. An asterisk denotes significant difference at p ≤ 0.05 (Two-way ANOVA with Tukey’s post-hoc test)