Table 3 Newly designed primers and clone libraries generated using these primers for sample S1
Target | Forward_primer | Reverse_primer | Annealing (°C) | Results of clone library |
|---|---|---|---|---|
Type A | 21AF: CTCTAGTTGATCCTGCTAGA | 1492R-22 W: TACGG(A/T)TACCTTGTTACGACTT | 47 | Euryarchaeota (26/28) b |
26AF: GG(A/G)CACTGCTATCGGCTT | 52 | Unclassified (14/14) | ||
59AF: AAGTCGAACGGACACGCAT | 52 | - a | ||
Type B | 12BF: CGATCCTGACGGAGCCTA | 1492R: GGTTACCTTGTTA(C/T)GACTT | 45 | – |
15BF: ATCCTGACGGAGCCTA | 45 | – | ||
16BF: TCCTGACGGAGCCTAC | 45 | – | ||
17BF: CCTGACGGAGCCTACT | 50 | Crenarchaeota (11/13) | ||
26BF: GCCTACTGCTATCGGATT | 45 | Thaumarchaeota (8/10) | ||
27BF: CCTACTGCTATCGGATT | 45 | Unclassified (8/9) | ||
Type A and Type B | 26ABF: G(G/C)(G/A/C)(C/T)ACT GCTATCGG(A/C)TT | 1492R: GGTTACCTTGTTA(C/T)GACTT | 50 | Unclassified (39/100) |