Skip to main content

Table 1 Summary of the evaluation of universal primers for meta-transcriptomic data of mudflat sediments [26], using MIPE

From: Design of targeted primers based on 16S rRNA sequences in meta-transcriptomic datasets and identification of a novel taxonomic group in the Asgard archaea

Domain

Primer

Incompletenessa

Completeness

   

matchingb

mismatching

    

No. mismatch = 1, 2

No. mismatch ≥3

Bacteria

8F

9603

452,840

12,664

894

Archaea

Arch21F

45

2060

1695

585

Unclassified

Arch21F

187

31

10

34

  1. a Number of reads that only contain incomplete binding sites for primers 8F or Arch21F
  2. b Reads completely matched with the primer or only contain 1 mismatch not at the last four nucleotides of the primer were considered matching; others, mismatching