Domain | Primer | Incompletenessa | Completeness |
---|
 |  |  | matchingb | mismatching |
---|
 |  |  |  | No. mismatch = 1, 2 | No. mismatch ≥3 |
Bacteria | 8F | 9603 | 452,840 | 12,664 | 894 |
Archaea | Arch21F | 45 | 2060 | 1695 | 585 |
Unclassified | Arch21F | 187 | 31 | 10 | 34 |
- a Number of reads that only contain incomplete binding sites for primers 8F or Arch21F
- b Reads completely matched with the primer or only contain 1 mismatch not at the last four nucleotides of the primer were considered matching; others, mismatching