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Fig. 2 | BMC Microbiology

Fig. 2

From: Design of targeted primers based on 16S rRNA sequences in meta-transcriptomic datasets and identification of a novel taxonomic group in the Asgard archaea

Fig. 2

Maximum likelihood (ML) phylogenetic tree for 16S rRNA gene sequences in the Asgard superphylum. Label 27BF refers to 16S rRNA gene clones amplified using primers 27BF and 1492R; Label 26AF, 26AF and modified 1492R. S1 and S2 labels indicate samples S1 and S2. PacBio sequences in this tree are representative operational taxonomic unit (OTU) sequences, and numbers of sequence labels in the parentheses represent the sizes of OTUs, and only OTUs ≥5 are shown herein. Two samples constituted phylum Thorarchaeota and a new group. Reference sequences were selected from SILVA_132 database, NCBI nucleotide Nr database, and genome taxonomy database (GTDB). Asgard-related 16S rRNA sequences reported by Karst et al. [28] are also included (labels start with OBEP). The bootstrap support value was set to 1000, and nodes above 0.75 are denoted by black circles. All alignment sequences are > 1200 bp. The scale bar indicates the number of substitutions per site

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BMC Microbiology

ISSN: 1471-2180

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