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Fig. 5 | BMC Microbiology

Fig. 5

From: An operon consisting of a P-type ATPase gene and a transcriptional regulator gene responsible for cadmium resistances in Bacillus vietamensis 151–6 and Bacillus marisflavi 151–25

Fig. 5

Genetic organization and transcription analysis of insert genes in fosmid clone B2 and examination of MIC-Cd for recombinant E. coli and B. subtilis containing the gene fragments of B. vietamensis 151–6. a Schematic representation of the insert fragments loci of fosmid clone B2. Thick gray line, B. vietamensis 151–6 chromosomal DNA; filled arrows and square, 31 open reading frames with corresponding gene size; black horizontal line, overexpressed gene fragments. b Transcription analysis of the insert genes in fosmid clone B2 in B. vietamensis 151–6 cultured with 0.1 mM Cd2+ (Cd) in comparison with a culture grown in the absence of Cd2+ (CK) using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Transcript levels of tested genes were normalized to the 16S rRNA gene. c Determination of MIC-Cd for recombinant E. coli containing the vectors pUC19 (negative control), 4087–4088-pUC19, 4093–4094-4095-pUC19, 4102–4103-pUC19, 4108–4109-pUC19 and 4111–4112-4113-pUC19 with varying concentrations of Cd2+ (0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6, 1.8, 2.0, 2.2, 2.4, 2.6, 2.8 and 3.0 mM). d Determination of MIC-Cd for recombinant B. subtilis containing the vectors pUBC19 (negative control), 4087–4088- pUBC19, 4093–4094-4095-pUBC19, 4102–4103-pUBC19, 4108–4109-pUBC19 and 4111–4112-4113-pUBC19 with varying concentrations of Cd2+ (0, 0.02, 0.04, 0.06, 0.08, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0 and 1.1 mM)

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