Fig. 4

Genetic organization and transcription analysis of up-regulated genes based on RNA sequencing and examination of MIC-Cd for recombinant E. coli and B. subtilis containing the gene fragments of p25. a Schematic representation of loci n the plasmid p25 fragment. Thick gray line, plasmid p25 DNA; filled arrows and squares, 19 open reading frames with corresponding gene sizes; black horizontal line, overexpressed gene fragments. b Transcription analysis of the plasmid p25 genes orf4774, 4775, 4776, 4777, 4779, 4781, 4782, 4802 and 4803 in B. marisflavi 151–25 cultured with 0.1 mM Cd²⁺ − Cd in comparison with a culture grown in the absence of Cd²⁺ (CK) using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Transcript levels of tested genes were normalized to the 16S rRNA gene. c Determination of MIC-Cd for recombinant E. coli containing the vectors pUC19 (negative control), 4774–4775-pUC19, 4776–4777-pUC19, 4779–4780-pUC19, 4781–4782-pUC19 and 4802–4803-pUC19 with varying concentrations of Cd²⁺ (0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6, 1.8, 2.0, 2.2, 2.4, 2.6, 2.8 and 3.0 mM). d Determination of MIC-Cd for recombinant B. subtilis containing the vectors pUBC19 (negative control), 4774–4775-pUBC19, 4776–4777-pUBC19, 4779–4780-pUBC19, 4781–4782-pUBC19 and 4802–4803-pUBC19 with varying concentrations of Cd²⁺ (0, 0.02, 0.04, 0.06, 0.08, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0 and 1.1 mM)