Fig. 5
From: Pneumococcal serotype determines growth and capsule size in human cerebrospinal fluid
Capsule sizes of strains after 9 h of incubation in BHI + FCS or hCSF. a FITC-dextran exclusion images of wild-type strain 106.66 serotype 6B (a,d) and of capsule switch mutant 106.66cps208.41 (serotype 7F) (b,e) and 106.66cps111.46 (serotype 19F) (c,f) and after 9 h of incubation in BHI + FCS (a,b,c) and hCSF (d,e,f). All images are to the same scale, taken using a 100X objective, and the scale bar indicates 10 μm. b and c show the mean area of bacterium (square pixels) after 9 h of incubation of wild-type strain 106.66 serotype 6B compared to its capsule switch mutants 106.66cps208.41 serotype 7F and 106.66cps111.46 serotype 19F in b BHI + FCS or c hCSF. No significant difference was observed between any of the strains in BHI + FCS. Significant differences were found when comparing 106.66 with 106.66cps208.41 (p-value = 0.0008) and 106.66cps111.46 (p-value < 0.0001) and when comparing 106.66cps208.41 and 106.66cps111.46 (0.026). Each symbol represents one image containing at least 2 bacteria. Error bars represent the standard error of the mean of three independent experiments