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Table 1 Experimental design (conditions and responses) for BPA degradation by Pseudomonas putida YC-AE1

From: Biodegradation of endocrine disruptor Bisphenol A by Pseudomonas putida strain YC-AE1 isolated from polluted soil, Guangdong, China

Run No. A B C D E BPA degradation (%)a
Initial pH (values) NaCl Conc. (%) Incubation period (hours) Inoculum size (%) Incubation temp. (°C)
7 5.00 (−2) 0.75 (0) 20 (0) 2.5 (0) 31 (0) 2.2
5 6.13 (−1) 1.13 (+ 1) 15 (− 1) 3.25 (+ 1) 37 (+ 1) 2.7
8 6.13 (−1) 1.13 (+ 1) 15 (−1) 1.75 (− 1) 25 (− 1) 7.8
10 6.13 (−1) 0.38 (− 1) 25 (+ 1) 1.75 (− 1) 25 (− 1) 81.7
11 6.13(− 1) 1.13 (+ 1) 25 (+ 1) 1.75 (− 1) 37 (+ 1) 0
12 6.13 (−1) 0.38 (− 1) 15 (− 1) 1.75 (− 1) 37 (+ 1) 0
14 6.13 (− 1) 1.13 (+ 1) 25 (+ 1) 3.25 (+ 1) 25 (− 1) 6.4
28 6.13 (−1) 0.38 (− 1) 25 (+ 1) 3.25 (+ 1) 37 (+ 1) 13.38
29 6.13 (−1) 0.38 (− 1) 15 (− 1) 3.25 (+ 1) 25 (− 1) 93.5
1 7.25 (0) 0.75 (0) 10 (−2) 2.5 (0) 31 (0) 2
2 7.25 (0) 1.5 (+ 2) 20 (0) 2.5 (0) 31 (0) 39.4
3 7.25 (0) 0.75 (0) 20 (0) 2.5 (0) 31 (0) 77
4 7.25 (0) 0.75 (0) 20 (0) 2.5 (0) 19 (−2) 17.8
15 7.25 (0) 0.75 (0) 20 (0) 2.5 (0) 31 (0) 77.2
16 7.25 (0) 0.75 (0) 30 (+ 2) 2.5 (0) 31 (0) 90
19 7.25 (0) 0.75 (0) 20 (0) 4 (+ 2) 31 (0) 63.2
20 7.25 (0) 0 (−2) 20 (0) 2.5 (0) 31 (0) 90
22 7.25 (0) 0.75 (0) 20 (0) 2.5 (0) 31 (0) 77.3
23 7.25 (0) 0.75 (0) 20 (0) 2.5 (0) 43 (+ 2) 0
24 7.25 (0) 0.75 (0) 20 (0) 1 (−2) 31 (0) 17.5
6 8.38 (+ 1) 0.38 (−1) 25 (+ 1) 1.75 (−1) 37 (+ 1) 1.7
9 8.38 (+ 1) 1.13 (+ 1) 15 (−1) 3.25 (+ 1) 25 (− 1) 17.5
13 8.38(+ 1) 1.13 (+ 1) 25 (+ 1) 1.75 (−1) 25 (− 1) 15
18 8.38(+ 1) 0.38 (−1) 15 (−1) 3.25 (+ 1) 37 (+ 1) 7
21 8.38 (+ 1) 0.38 (−1) 25 (+ 1) 3.25 (+ 1) 25 (− 1) 95
25 8.38 (+ 1) 1.13 (+ 1) 15 (−1) 1.75 (− 1) 37 (+ 1) 0
26 8.38 (+ 1) 1.13 (+ 1) 25 (+ 1) 3.25 (+ 1) 37 (+ 1) 0
27 8.38 (+ 1) 0.38 (−1) 15 (− 1) 1.75 (− 1) 25 (− 1) 78.3
17 9.5 (+ 2) 0.75 (0) 20 (0) 2.5 (0) 31 (0) 60
  1. aAll response (BPA degradation percentage) values are shown as means for three independent experiments