Fig. 4
Effect of antibiotic therapy on Galleria mellonella larva survival after injection with Fno. Kaplan-Meier plots of G. mellonella larva survival during 264 h at 28 °C after injection of ca. 1 × 109 CFU/mL of (a) Fno STIR-GUS-F2f7, (b) Fno Austria, (c) Fno PQ1104, (d) Fno Franc-COS1, and (e) Fno Ehime-1, and treatment with tetracycline at 10 mg/g body weight at 2, 24 and 48 h (infected +TET), showing that the antibiotic treatment increased larval survival compared to groups treated with PBS (infected -TET). Actual CFU/mL after plating bacterial suspensions of each Fno isolate on CHAH were: 0.81–1.33 × 109 for Fno STIR-GUS-F2f7, 0.81–1.25 × 109 for Fno Austria, 0.90–1.10 × 109 for Fno PQ1104, 1.32–1.35 × 109 for Fno Franc-COS1, and 0.87–0.90 × 109 for Fno Ehime-1. One control group of larvae was injected with PBS instead of bacteria and treated with the tetracycline (TET only) to assess the toxicity of the antibiotic alone, while survival was 100% in the unmanipulated and PBS only control groups for each replicate (data not shown). n = 24