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Fig. 7 | BMC Microbiology

Fig. 7

From: Revealing biophysical properties of KfrA-type proteins as a novel class of cytoskeletal, coiled-coil plasmid-encoded proteins

Fig. 7

Images of KfrA proteins in the presence of specific DNA binding sites. a KfrA proteins at concentration 0.3 mg ml− 1 in 50 mM sodium phosphate pH 7.5, 150 mM NaCl buffer were visualized by transmission electron microscopy (TEM) without plasmid DNA or with pUC18 DNA as the control (12.5 μg ml− 1). b Filaments of KfrAR751 and KfrARA3 were formed (as indicated by black arrows) after incubation with pUC18 derivatives, respectively pMAB18.7/8 and pESB2.68, carrying native, specific OK motifs. Inset shows magnified view of the braided fibrillar structure, the scale bar - 50 nm

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