Fig. 6
DNA binding activity of KfrA proteins from R751 and RA3 plasmids assayed by EMSA. Appropriate His-tagged KfrA proteins were incubated for 30 min at 25 °C with 10 pmols of fluorescently labelled ds oligonucleotides corresponding to OKR751 (oligonucleotides #15/#16 labelled with Cy5) or OKRA3 (#17/#18 labelled with Cy3). Double stranded oligonucleotides (annealed #5 and #6) were used as nonspecific DNA in binding reactions separated on 2.5% agarose gels run in 0.5xTBE. The right panel for KfrARA3 demonstrates the results when the increasing quantities of unlabeled competitive ds oligonucleotide (annealed #9 and #10 oligonucleotides) was added to the binding reactions