Altered gut microbiota correlate with different immune responses to HAART in HIV-infected individuals

Xie, Yirui; Sun, Jia; Wei, Li; Jiang, Haiyin; Hu, Caiqin; Yang, Jiezuan; Huang, Ying; Ruan, Bing; Zhu, Biao

doi:10.1186/s12866-020-02074-1

BMC Microbiology

Table 2 Cellular immune activation markers sequencing data summary

From: Altered gut microbiota correlate with different immune responses to HAART in HIV-infected individuals

T cell markers		HIV ART (+)		P-value
T cell markers	Health Control	Immune Responders (IR)	Immune Non-responders (INR)	P-value
Nadir CD4⁺ T cells (mean ± SD)	NA	309.89 ± 128.81	95.23 ± 108.92	< 0.0001
Current CD4 ⁺ T cells (mean ± SD)	NA	608.30 ± 158.25	230.5 ± 87.50	< 0.0001
Current CD4+/CD8+ T-cell ratio	NA	0.8 ± 0.36	0.35 ± 0.20	< 0.0001
HIV RNA	NA	ND	ND
%CD4 + HLADR+CD38+	NA	7.72 ± 4.30	10.30 ± 10.45	NS
%CD4 + CD25+	NA	1.26 ± 0.99	1.09 ± 1.04	NS
%CD4 + CD57+	NA	2.57 ± 1.92	2.43 ± 3.30	NS
%CD8 + HLADR+CD38+	NA	20.68 ± 11.35	23 ± 12.39	NS
%CD8 + CD57+	NA	14.36 ± 7.11	23.98 ± 12.30	0.001
Cytokines
IL-2(pg/mL, mean ± SD)	9.47 ± 6.79	120.96 ± 113.96	153.81 ± 118.79	< 0.0001
IL-4(pg/mL, mean ± SD)	13.49 ± 31.01	34.52 ± 43.30	32.51 ± 31.17	0.009
IL-6(pg/mL, mean ± SD)	8.39 ± 12.57	126.90 ± 95.33	130.82 ± 60.62	< 0.0001
IL-9(pg/mL, mean ± SD)	3.68 ± 6.20	94.00 ± 78.07	96.57 ± 48.71	< 0.0001
IL10(pg/mL, mean ± SD)	2.48 ± 2.35	44.69 ± 37.66	51.32 ± 31.65	< 0.0001
IL-13(pg/mL, mean ± SD)	85.45 ± 65.02	96.76 ± 71.54	82.94 ± 62.96	NS
IL-17A (pg/mL, mean ± SD)	26.22 ± 52.89	133.41 ± 161.61	94.80 ± 83.84	< 0.0001
IL-17F (pg/mL, mean ± SD)	5.45 ± 5.68	19.15 ± 19.48	20.59 ± 20.34	< 0.0001
IL-21(pg/mL, mean ± SD)	41.33 ± 48.87	104.43 ± 88.11	93.84 ± 56.89	0.002
IL-22(pg/mL, mean ± SD)	153.98 ± 97.15	291.60 ± 176.36	293.10 ± 125.37	0.001
IFN-γ (pg/mL, mean ± SD)	12.55 ± 29.52	81.57 ± 84.50	69.36 ± 83.42	< 0.0001
TNF-α (pg/mL, mean ± SD)	8.39 ± 15.57	38.86 ± 44.19	32.22 ± 26.47	< 0.0001
LPS (pg/mL, mean ± SD)	24.22 ± 18.88	76.55 ± 40.05	104.98 ± 56.15	< 0.0001^b
sCD14(pg/mL, mean ± SD)	1583.60 ± 292.80	2480.42 ± 999.88	2142.65 ± 496.53	< 0.0001
Pyrosequencing data
Sobs index^a	194.11 ± 47.45	116.89 ± 39.71	118.11 ± 46.96	< 0.0001
Shannon index^a	3.14 ± 0.53	2.47 ± 0.54	2.44 ± 0.56	< 0.0001
Simpson index^a	0.12 ± 0.09	0.19 ± 0.11	0.19 ± 0.13	< 0.0001
ACE^a	223.97 ± 53.59	147.26 ± 39.98	148.41 ± 49.99	< 0.0001
Chao 1 index^a	224.29 ± 54.76	144.52 ± 45.25	145.37 ± 51.01	< 0.0001
Good’s coverage (%) ^a	99.82 ± 0.04	99.87 ± 0.04	99.87 ± 0.04	< 0.0001

^aIndicates that the alpha diversity was calculated after the reads number of each sample were equalized. NA (not available), ND (not detected), NS (no significant) indicates p-value > 0.05, ^bcompared between the IR and the INR group

Back to article page

ISSN: 1471-2180

Contact us

General enquiries: journalsubmissions@springernature.com