Table 1 Differentially secreted proteins of planktonic and biofilm-cultured ML09–119

 DNA gyrase inhibitor

AHML_21105

2.2

0.01

0

16^a

BIO

 Ribonuclease activity regulator

AHML_16315

1.55

0.02

0.02

2.5

BIO

 FKBP-type peptidyl-prolyl cis-trans isomerase

AHML_05355

−2.10

0.01

0

22^a

TSB

 Cytochrome d ubiquinol oxidase

AHML_19200

−1.71

0.02

0.02

16

TSB

 Superoxide dismutase

AHML_07590

2.95

0.01

0

6

BIO

 Glyoxalase/dioxygenase protein

AHML_09045

1.89

0.01

0.03

13^a

BIO

 Redox protein (hypothetical)

AHML_05250

2.33

0.01

0

19^a

BIO

 Diaminopimelate epimerase

AHML_02440

1.82

0.02

0.03

9^a

BIO

 Ornithine carbamoyltransferase

AHML_21555

−1.35

0.03

0.05

3.5

TSB

 Riboflavin-biosynthesis protein

AHML_17795

1.4

0.02

0.05

2.4

BIO

 Urocanate hydratase

AHML_01870

3.71

0.00

0

8

BIO

 Dihydrodipicolinate synthase

AHML_04540

2.35

0.01

0

22^a

BIO

 Succinylarginine dihydrolase

AHML_16715

5.49

0.00

0

66^a

BIO

 Maltose operon periplasmic protein

AHML_06220

2.57

0.01

0

15^a

BIO

 Phosphoglyceromutase

AHML_01445

1.94

0.01

0

13^a

BIO

 Transaldolase B

AHML_16890

8.08

0.00

0

89^a

BIO

 Pullulanase

AHML_04415

3.62

0.00

0

10

BIO

 Ribose-5-phosphate isomerase A

AHML_14480

2.92

0.01

0

3

BIO

 Beta-glucosidase

AHML_14270

−1.68

0.02

0.0

14

TSB

 Outer membrane protein A

AHML_21905

−1.99

0.01

0

4

TSB

 Outer membrane protein A

AHML_20145

1.46

0.02

0.04

13^a

BIO

 Outer membrane lipoprotein

AHML_00700

−1.58

0.02

0.02

13^a

TSB

 Hemolysin (Aerolysin-type)

AHML_02265

−2.37

0.01

0

3

TSB

 Hemolysin (ahh1-type)

AHML_08400

−2.69

0.01

0

4

TSB

 Elastase

AHML_04340

4.68

0.00

0

5

BIO

 Chitinase

AHML_05225

4.07

0.00

0

3

BIO

 Metalloprotease

AHML_05230

2.73

0.01

0

3

BIO

 Basic endochitinase

AHML_05235

3.15

0.01

0

3

BIO

 Zn-dependent carboxypeptidase

AHML_05535

2.15

0.01

0

13^a

BIO

 Outer membrane porin protein

AHML_04355

2.91

0.01

0

15^a

BIO

 Extracellular lipase

AHML_00550

−4.16

0.01

0

3

TSB

 Chitin-binding domain 3

AHML_11110

−1.50

0.02

0.02

12

TSB

 Zn-dependent protease with chaperone function

AHML_06635

1.51

0.02

0.02

11^a

BIO

 Phosphoglyceromutase

AHML_01445

1.94

0.01

0

13^a

BIO

 Phospholipid-cholesterol acyltransferase

AHML_20135

−1.55

0.02

0.02

14^a

TSB

 Aminopeptidase

AHML_18480

−1.55

0.02

0.02

2

TSB

 Chitin binding protein

AHML_03125

1.59

0.02

0.02

1.6

BIO

 Proline iminopeptidase

AHML_18440

1.88

0.01

0.03

14^a

BIO

 Serine Protease

AHML_14260

−1.91

0.01

0.03

19

TSB

 Periplasmic carboxy-terminal protease

AHML_11480

2.28

0.01

0

14^a

BIO

 Collagenase family

AHML_02655

−1.90

0.01

0.03

2.5

TSB

 Extracellular alkaline serine protease

AHML_18455

−2.22

0.01

0

25^a

TSB

 Small protease

AHML_08855

1.37

0.03

0.05

8^a

BIO

 Flagellin

AHML_09350

5.42

0.00

0

43^a

BIO

 Flagellin-like protein

AHML_09345

2.05

0.01

0

2.6

BIO

 Type I pilus assembly protein FimF

AHML_02690

−2.29

0.01

0

26^a

TSB

 Fimbrial Proteain

AHML_02665

−2.61

0.01

0

36^a

TSB

 ABC-type sugar transport

AHML_20895

1.77

0.02

0.02

14^a

BIO

 TonB-dependent copper receptor

AHML_02545

1.79

0.02

0.02

9

BIO

 Peptide ABC transporter

AHML_17755

3.2

0.00

0

98^a

BIO

 Arginine ABC transporter

AHML_03370

4.58

0.00

0

7

BIO

 Oligopeptide ABC transporter

AHML_13875

6.05

0.00

0

93^a

BIO

 Leucine binding protein (ABC transport)

AHML_00595

3.74

0.00

0

18

BIO