Fig. 4From: Differential production and secretion of potentially toxigenic extracellular proteins from hypervirulent Aeromonas hydrophila under biofilm and planktonic cultureGross lesions and histopathology of channel catfish muscle following intramuscular injection with vAh secreted proteins. (A) Channel catfish injected with Control (top) and biofilm-cultured vAh secreted proteins (bottom) 24 h post-injection, with black circle denoting injection site. Histologic sections prepared from paraffin-embedded tissues were stained with hematotoxylin and eosin. (B) Control (200X) – No perceptible damage to skin, subcutaneous adipose tissue, or muscle. Fish injected with planktonic ECPs were indistinguishable from controls. (C) Biofilm-injected fish tissue (200X) 24 h post-injection. Tissue was edematous, hemorrhagic, and necrotic at the injection site. Despite substantial tissue damage, few inflammatory cells were presentBack to article page