Galectins from Onchocerca ochengi and O. volvulus and their immune recognition by Wistar rats, Gudali zebu cattle and human hosts

Table 2 Peripheral blood mononuclear cells from cattle with different parasitic loads lack response to O. ochengi galectin and O. ochengi worm extract

Antigens	OD of animals with low/high nodules load (NL)		OD of animals with low/high Mff density (MD)
	Median (IQR)		Median (IQR)
	Low	High	Low	High
Control (culture medium)	0.119 (0.096–0.123)	0.125 (0.079–0.133)	0.119 (0.101–0.174)	0.084 (0.079–0.126)
O. ochengi galectin	0.133 (0.097–0.151)	0.133 (0.095–0.166)	0.151 (0.105–0.227)	0.099 (0.092–0.146)
O. ochengi extract	0.099 (0.083–0.118)	0.098 (0.083–0.127)	0.104 (0.083–0.154)	0.085 (0.082–0.109)
ConA.	1.275 (0.618–1.570)	1.364 (0.279–2.094	1.289 (0.618–1.531)	1.056 (0.279–1.725)
Control: low vs high (NL or MD)	P = 0.754		P = 0.251
O. ochengi galectin: low vs high (NL or MD)	P = 0.675		P = 0.402
O. ochengi extract: low vs high (NL or MD)	P = 0.917		P = 0.251
ConA: low vs high (NL or MD)	P = 0.0754		P = 0.754

Mann-Whitney U test analysis of the proliferation of PBMCs from cattle using cattle with low and high parasite loads (low parasite load: 0–16 nodules, 0 mff/mg,of skin, high parasite load: 103–920 nodules, 8–21 mff/mg of skin), n = 5 per group. PBMCS were exposed for 3 days to O. ochengi galectin, O. ochengi extract from females worms and to polyclonal activator ConA as positive control. Applying the MTT proliferation assay the absorbance of the MTT was measured as optical density (OD). IQR interquartile range of OD indicated in brackets

ISSN: 1471-2180