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Fig. 1 | BMC Microbiology

Fig. 1

From: Galectins from Onchocerca ochengi and O. volvulus and their immune recognition by Wistar rats, Gudali zebu cattle and human hosts

Fig. 1

Amplification products of O. ochengi cDNA transcribed from RNA isolated from O. ochengi worm stages. a: Amplification products of O. ochengi cDNA transcribed from RNA which was isolated from O. ochengi adult female and male worms, uterine and skin microfilariae, and L3 as templates on 1% agarose gel. The length of the cDNA is about 850 bp. b: Course of affinity chromatography purification of O. ochengi recombinant galectin using Ni-NTA resin and selected fractions ran on a 12% SDS-PAGE gel. c: corresponding western blot using anti-His antibody. The lanes show: Marker: standard proteins, lane S1: supernatant before binding to beads, lane S2: supernatant after binding to beads, lanes W1, W2, W3: wash fractions, lanes E1, E2, E3: eluted fractions of recombinant O. ochengi galectin. E3 shows a decrease in the amount of recombinant O. ochengi galectin present in sample. Three separate elutions (E1, E2 and E3) separated by an interval of 10 min were undertaken to maximize the quantity of eluted proteins

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