Table 1 Primers used in qPCR studies
Primer name | Sequence | #nucleotides | Amplicon length (bp) | Target gene | Reference |
|---|---|---|---|---|---|
Universal 16S, F | ACTCCTACGGGAGGCAGCAGT | 21 | 192 | 16S rRNA | Scher et al., 2013 [12] |
Universal 16S, F | ATTACCGCGGCTGCTGGC | 18 | |||
P. copri genus 16S, F | CACRGTAAACGATGGATGCC | 20 | 516 | 16S rRNA | Matsuki et al., 2002 |
P. copri genus 16S, R | GGTCGGGTTGCAGACC | 16 | |||
P. copri genome specific, F | CCGGACTCCTGCCCCTGCAA | 20 | 106 | FimB/Mfa2 family fimbrial subunit | Scher et al., 2013 [12] |
P. copri genome specific, R | GTTGCGCCAGGCACTGCGAT | 20 | PREVCOP_RS00015 | ||
P. copri 16S, 1F | ACATCGAAAGCTTGCTTTTG | 20 | 409 | 16S rRNA | This publication |
P. copri 16S, 1R | CAAAAAGCCTCACGAGGCTC | 20 | AB064923 | ||
P. copri 16S, 2F | ACCACTTGGGGATAACCTTG | 20 | 347 | 16S rRNA | This publication |
P. copri 16S, 2R | TACATGCAAAAAGCCTCACGAGGC | 24 | AB064923 | ||
P. copri 16S, 3F | TCTCTAGAAGACATCTGAAAGA | 22 | 446 | 16S rRNA | This publication |
P. copri 16S, 3R | CAGTGCAGACGTTGAGCGT | 19 | AB064923 | ||
P. copri16S, 4F | CGAAAGCTTGCTTTTGATGG | 20 | 86 | 16S rRNA | This publication |
P. copri 16S, 4R | CGCAAGGTTATCCCCAAGT | 19 | AB064923 | ||
P. copri genome specific, 1F | TTTTGCTGTAGGAGGGGTTG | 20 | 96 | glycosyl transferase, group 1 | This publication |
P. copri genome specific, 1R | GGGCTGCATAAAGCAAAGAC | 20 | PREVCOP_06806 | ||
P. copri genome specific, 2F | AGCCGAGATATCGTGAGTGG | 20 | 137 | RNA polymerase ECF-type sigma factor | This publication |
P. copri genome specific, 2R | TGAACAGCTGTATGCCGAAG | 20 | PREVCOP_06715 | ||
P. copri genome specific, 3F | AGTTTGTCAATGCCCTCCTG | 20 | 141 | CAAX amino protease family protein | This publication |
P. copri genome specific, 3R | CATCGCTCTGAGGCATGATA | 20 | PREVCOP_06538 | ||
P. copri genome specific, 4F | TCGCTGACATGAGCGATAAC | 20 | 109 | metalloprotease domain protein, M6 family | This publication |
P. copri genome specific, 4R | CCGTTGGCACTACCTTCATT | 20 | PREVCOP_04242 |