Table 2 Primers and probe sequences targeting ITS-2 region of ribosome DNA in A. tetraptera
Primer/Probe | Sequence | Tm (°C) | Product size (bp) |
|---|---|---|---|
Normal oligo primer Fw | ATACTCTTTGACGCATACACAC | 58.2 | 75 |
Normal oligo primer Rv | CAGCCATAGGTATTGTTATATGAGC | 61.4 | |
LNA oligo primer Fw | ATACTCTTTGACGCATACACAC | 63 | 75 |
LNA oligo primer Rv | CAGCCATAGGTATTGTTATATGAGC | 63 | |
LNA TaqMan probe | FAM/CATACACACCGTATATGTGTTGC/3IABkFQ | 57 | – |