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Table 1 Ct values in the amplification of positive control DNA with each primer concentration

From: Standardization of an LNA-based TaqMan assay qPCR analysis for Aspiculuris tetraptera DNA in mouse faeces

Assay Primer Copies Primer conc.
75 nM 37.5 nM 18.8 nM 9.4 nM
SYBR Green Normal 0 36.5 ± 0.59 n.d n.d n.d
100 21.6 ± 0.75 32.6 ± 1.66 43.9 ± 1.13 n.d
LNA 0 39.3 ± 1.45 40.8 ± 0.68 44.5 ± 0.46 n.d
100 21.9 ± 1.98 23.3 ± 0.73 36.4 ± 1.39 n.d
Taqman LNA probe Normal 0 31.4 ± 1.57 34.8 ± 3.3 34.4 ± 0.73 42.1 ± 1.56
100 15.7 ± 0.16 17.9 ± 1.31 18.9 ± 0.61 28.3 ± 4.19
LNA 0 35.1 ± 3.16 30.4 ± 0.23 32.5 ± 0.52 40.2 ± 2.23
100 17.6 ± 1.5 16.2 ± 0.23 18.3 ± 0.61 24.8 ± 2.41
  1. Mean ± SE