Table 1 Ct values in the amplification of positive control DNA with each primer concentration
Assay | Primer | Copies | Primer conc. | |||
|---|---|---|---|---|---|---|
75 nM | 37.5 nM | 18.8 nM | 9.4 nM | |||
SYBR Green | Normal | 0 | 36.5 ± 0.59 | n.d | n.d | n.d |
100 | 21.6 ± 0.75 | 32.6 ± 1.66 | 43.9 ± 1.13 | n.d | ||
LNA | 0 | 39.3 ± 1.45 | 40.8 ± 0.68 | 44.5 ± 0.46 | n.d | |
100 | 21.9 ± 1.98 | 23.3 ± 0.73 | 36.4 ± 1.39 | n.d | ||
Taqman LNA probe | Normal | 0 | 31.4 ± 1.57 | 34.8 ± 3.3 | 34.4 ± 0.73 | 42.1 ± 1.56 |
100 | 15.7 ± 0.16 | 17.9 ± 1.31 | 18.9 ± 0.61 | 28.3 ± 4.19 | ||
LNA | 0 | 35.1 ± 3.16 | 30.4 ± 0.23 | 32.5 ± 0.52 | 40.2 ± 2.23 | |
100 | 17.6 ± 1.5 | 16.2 ± 0.23 | 18.3 ± 0.61 | 24.8 ± 2.41 | ||