Skip to main content
Fig. 1 | BMC Microbiology

Fig. 1

From: Standardization of an LNA-based TaqMan assay qPCR analysis for Aspiculuris tetraptera DNA in mouse faeces

Fig. 1

Optimizing PCR experimental conditions to detect A. tetraptera DNA. a-c Synthesized A. tetraptera DNA was amplified with Taq polymerase, as described in the “Materials and methods” section, at the indicated annealing temperature (a) and primer concentrations (b) using the indicated cycle numbers (c), and the products were subsequently observed by agarose gel electrophoresis using ethidium bromide staining. d The bands of the PCR products were quantified using the Metamorph imaging software. The signal intensity was expressed by division of total gray value by number of pixels, and corrected by background intensities. The correlation coefficient indicates the relationship between the band intensity and the copy number of synthesized A. tetraptera DNA in the range from 10 to 1000 copies. Data represent the mean obtained from three independent experiments. The genomic DNA were purified from the faeces of A. tetraptera-positive mice and amplified using the indicated amounts of genomic DNA under the optimal PCR experimental conditions. The PCR products were visualized by agarose gel electrophoresis using ethidium bromide staining (e), and the bands were quantified using the Metamorph imaging software (f). The data represent the mean ± SE obtained from three independent experiments. PC shows the data of the synthesized A. tetraptera DNA in the absence of genomic DNA

Back to article page