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Fig. 5 | BMC Microbiology

Fig. 5

From: Role of aspartate ammonia-lyase in Pasteurella multocida

Fig. 5

AspA is required for iron acquisition. a Effect of DPD supplementation on C48–1 and aspA::kan growth under aerobic conditions. The final OD600 values were determined after incubation in TSB in different iron-limited conditions at 16 h. b The growth curves of C48–1, aspA::kan, CaspA::kan and aspA::kan (pAL99S) were measured at 37° in TSB containing 150 μM DPD under aerobic conditions. c Utilization of iron sources by C48–1 and aspA::kan, CaspA::kan and aspA::kan (pAL99S). Wild-type and aspA mutant strains were inoculated in TSB containing 150 μM DPD supplemented with FeCl3 or FeSO4 under aerobic conditions. The final OD600 values were determined after 16 h. d Transcription levels of the aspA gene in C48–1, aspA::kan and CaspA::kan in response to different iron-limited conditions. Data analyzed by Student’s t-test. * P < 0.05; ** P < 0.01 and NS for P>0.05

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