Fig. 3
Inhibitory activities of four organic extracts of 14 bacterial isolates against PLA2 enzyme and their correlation with bacterial pathogenicity. Bacteria used in this assay included 14 isolates: Photorhabdus temperata Subsp. temperata ANU101 (‘Ptt’), Xenorhabdus hominickii ANU101 (‘Xh’), X. nematophila K1 (‘XnK1’), X. ehlersii KSY (‘Xe’),, X. nematophila SK1 (‘XnSK1’), X. nematophila SK2 (‘XnSK2’), Photorhabdus luminescens KACC12123 (‘Pl 193’), P. luminescens subsp. laumondii KACC12283 (‘Pl laum’), P. luminescens subsp. thracensis KACC12284 (‘Pl thra’), X. nematophila KACC12145 (‘Xn12145’), X. nematophila Mexico (‘XnM’), X. nematophila France (‘XnF’), X. bovienii (‘Xb’), and X. poinarii (‘Xp’). Their cultured broths were extracted with four different organic solvents: hexane (‘HEX’), ethyl acetate (‘EAX’), chloroform (‘CX’), and butanol (‘BX’). a Effects of organic extracts on sPLA2. b Effects of organic extracts on cPLA2. DMSO was used as control. Each treatment was replicated three times using independent samples. Different letters above standard deviation bars indicate significant differences among means at Type I error = 0.05 (LSD test). c Correlations (r) between insecticidal activities of bacterial extracts (Fig. 1) and their inhibitory activities against PLA2 enzyme. Lines represent the best-fit regression