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Table 3 The level of alkaloids and nitrate of tobacco leaves in different groups

From: Influence of microbiota and metabolites on the quality of tobacco during fermentation

Groups Nicotine (μg/g) Nornicotine (μg/g) Neonicotine (μg/g) Anatabine (μg/g) Total alkaloids (%) Nitrate (μg/g)
Control 5.16 ± 0.07 0.24 ± 0.01 0.05 ± 0.00 0.48 ± 0.02 5.92 ± 0.10Aa 3589.33 ± 52.78Aa
25 °C/ 2 weeks 4.90 ± 0.07 0.21 ± 0.01 0.04 ± 0.00 0.41 ± 0.02 5.56 ± 0.10Bb 3354.15 ± 52.20Bb
25 °C/ 4 weeks 4.69 ± 0.08 0.19 ± 0.01 0.04 ± 0.00 0.41 ± 0.02 5.33 ± 0.10Bc 3160.14 ± 52.29Ccd
25 °C/ 6 weeks 4.24 ± 0.08 0.18 ± 0.01 0.03 ± 0.00 0.36 ± 0.02 4.82 ± 0.12CDde 2991.19 ± 54.31DEe
35 °C/ 2 weeks 4.35 ± 0.08 0.20 ± 0.01 0.03 ± 0.00 0.40 ± 0.02 4.98 ± 0.11Cd 3210.28 ± 56.96Cc
35 °C/ 4 weeks 4.25 ± 0.08 0.18 ± 0.01 0.03 ± 0.00 0.38 ± 0.02 4.85 ± 0.12CDde 2938.96 ± 55.11Ee
35 °C/ 6 weeks 4.11 ± 0.06 0.16 ± 0.01 0.03 ± 0.00 0.35 ± 0.02 4.65 ± 0.12DEef 2674.46 ± 58.02FGg
45 °C/ 2 weeks 4.31 ± 0.09 0.19 ± 0.01 0.03 ± 0.00 0.38 ± 0.02 4.91 ± 0.10CDd 3094.13 ± 51.96CDd
45 °C/ 4 weeks 4.20 ± 0.08 0.18 ± 0.01 0.03 ± 0.00 0.37 ± 0.02 4.78 ± 0.13CDde 2785.81 ± 57.53Ff
45 °C/ 6 weeks 3.96 ± 0.06 0.16 ± 0.01 0.03 ± 0.00 0.35 ± 0.02 4.50 ± 0.12Ef 2569.76 ± 53.03Gh
  1. Control group represents the sun-dried unfermented tobacco leaves. All data are represented by mean ± SD (n = 3). Uppercase letters indicate p-value < 0.05 and lowercase letters indicate p-value < 0.01