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Table 3 The level of alkaloids and nitrate of tobacco leaves in different groups

From: Influence of microbiota and metabolites on the quality of tobacco during fermentation

Groups

Nicotine (μg/g)

Nornicotine (μg/g)

Neonicotine (μg/g)

Anatabine (μg/g)

Total alkaloids (%)

Nitrate (μg/g)

Control

5.16 ± 0.07

0.24 ± 0.01

0.05 ± 0.00

0.48 ± 0.02

5.92 ± 0.10Aa

3589.33 ± 52.78Aa

25 °C/ 2 weeks

4.90 ± 0.07

0.21 ± 0.01

0.04 ± 0.00

0.41 ± 0.02

5.56 ± 0.10Bb

3354.15 ± 52.20Bb

25 °C/ 4 weeks

4.69 ± 0.08

0.19 ± 0.01

0.04 ± 0.00

0.41 ± 0.02

5.33 ± 0.10Bc

3160.14 ± 52.29Ccd

25 °C/ 6 weeks

4.24 ± 0.08

0.18 ± 0.01

0.03 ± 0.00

0.36 ± 0.02

4.82 ± 0.12CDde

2991.19 ± 54.31DEe

35 °C/ 2 weeks

4.35 ± 0.08

0.20 ± 0.01

0.03 ± 0.00

0.40 ± 0.02

4.98 ± 0.11Cd

3210.28 ± 56.96Cc

35 °C/ 4 weeks

4.25 ± 0.08

0.18 ± 0.01

0.03 ± 0.00

0.38 ± 0.02

4.85 ± 0.12CDde

2938.96 ± 55.11Ee

35 °C/ 6 weeks

4.11 ± 0.06

0.16 ± 0.01

0.03 ± 0.00

0.35 ± 0.02

4.65 ± 0.12DEef

2674.46 ± 58.02FGg

45 °C/ 2 weeks

4.31 ± 0.09

0.19 ± 0.01

0.03 ± 0.00

0.38 ± 0.02

4.91 ± 0.10CDd

3094.13 ± 51.96CDd

45 °C/ 4 weeks

4.20 ± 0.08

0.18 ± 0.01

0.03 ± 0.00

0.37 ± 0.02

4.78 ± 0.13CDde

2785.81 ± 57.53Ff

45 °C/ 6 weeks

3.96 ± 0.06

0.16 ± 0.01

0.03 ± 0.00

0.35 ± 0.02

4.50 ± 0.12Ef

2569.76 ± 53.03Gh

  1. Control group represents the sun-dried unfermented tobacco leaves. All data are represented by mean ± SD (n = 3). Uppercase letters indicate p-value < 0.05 and lowercase letters indicate p-value < 0.01