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Table 2 The level of NNN, NAT, NAB, NNK, and TSNAs in tobacco leaves during fermentation process

From: Influence of microbiota and metabolites on the quality of tobacco during fermentation

Groups

NNN (ng/g)

NAT (ng/g)

NAB (ng/g)

NNK (ng/g)

TSNAs (ng/g)

Control

812.73 ± 33.2Gg

1459.82 ± 84.60Ee

40.30 ± 0.37Hh

145.34 ± 8.07Ef

2458.19 ± 126.36Gf

25 °C/ 2 weeks

934.19 ± 31.32Ff

2060.91 ± 85.57Dd

63.81 ± 0.39Gg

242.30 ± 8.13De

3301.21 ± 125.41Fe

25 °C/ 4 weeks

1159.46 ± 33.55Ee

2274.54 ± 83.56Cc

72.49 ± 0.36Ff

293.61 ± 8.41Cd

3800.10 ± 125.88Ed

25 °C/ 6 weeks

1546.99 ± 36.10Bb

2536.65 ± 88.18ABb

84.10 ± 0.32Dd

349.86 ± 8.47ABb

4517.60 ± 133.06BCb

35 °C/ 2 weeks

976.51 ± 36.06Ff

2069.04 ± 88.04Dd

72.81 ± 0.35Ff

246.66 ± 8.22De

3365.03 ± 132.67Fe

35 °C/ 4 weeks

1251.87 ± 35.24Dd

2394.27 ± 84.81BCbc

84.34 ± 0.30Dd

300.26 ± 8.37Cd

4030.73 ± 128.72DEd

35 °C/ 6 weeks

1602.03 ± 39.96Bb

2694.59 ± 80.05Aa

90.19 ± 0.35Bb

354.84 ± 8.07ABab

4741.66 ± 128.43ABa

45 °C/ 2 weeks

1192.84 ± 33.58DEde

2294.08 ± 89.99Cc

73.86 ± 0.34Ee

287.15 ± 8.22Cd

3847.94 ± 132.12Ed

45 °C/ 4 weeks

1439.20 ± 31.50Cc

2407.34 ± 89.62BCbc

86.59 ± 0.36Cc

335.67 ± 8.30Bc

4268.80 ± 129.78CDc

45 °C/ 6 weeks

1685.79 ± 38.24Aa

2720.40 ± 84.92Aa

95.80 ± 0.34Aa

367.12 ± 8.14Aa

4869.12 ± 131.63Aa

  1. NNN N′-nitrosonornicotine; NAT N′-nitrosoanatabine; NAB N′-nitrosoanabasine; NNK 4-(methylnitrosamino)-1 -(3-pyridyl)-1-butanone; TSNAs Tobacco-specific Nitrosamines. Control group represents the sun-dried unfermented tobacco leaves. All data are represented by mean ± SD (n = 3). Uppercase letters indicate p-value < 0.05 and lowercase letters indicate p-value < 0.01