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Table 2 The level of NNN, NAT, NAB, NNK, and TSNAs in tobacco leaves during fermentation process

From: Influence of microbiota and metabolites on the quality of tobacco during fermentation

Groups NNN (ng/g) NAT (ng/g) NAB (ng/g) NNK (ng/g) TSNAs (ng/g)
Control 812.73 ± 33.2Gg 1459.82 ± 84.60Ee 40.30 ± 0.37Hh 145.34 ± 8.07Ef 2458.19 ± 126.36Gf
25 °C/ 2 weeks 934.19 ± 31.32Ff 2060.91 ± 85.57Dd 63.81 ± 0.39Gg 242.30 ± 8.13De 3301.21 ± 125.41Fe
25 °C/ 4 weeks 1159.46 ± 33.55Ee 2274.54 ± 83.56Cc 72.49 ± 0.36Ff 293.61 ± 8.41Cd 3800.10 ± 125.88Ed
25 °C/ 6 weeks 1546.99 ± 36.10Bb 2536.65 ± 88.18ABb 84.10 ± 0.32Dd 349.86 ± 8.47ABb 4517.60 ± 133.06BCb
35 °C/ 2 weeks 976.51 ± 36.06Ff 2069.04 ± 88.04Dd 72.81 ± 0.35Ff 246.66 ± 8.22De 3365.03 ± 132.67Fe
35 °C/ 4 weeks 1251.87 ± 35.24Dd 2394.27 ± 84.81BCbc 84.34 ± 0.30Dd 300.26 ± 8.37Cd 4030.73 ± 128.72DEd
35 °C/ 6 weeks 1602.03 ± 39.96Bb 2694.59 ± 80.05Aa 90.19 ± 0.35Bb 354.84 ± 8.07ABab 4741.66 ± 128.43ABa
45 °C/ 2 weeks 1192.84 ± 33.58DEde 2294.08 ± 89.99Cc 73.86 ± 0.34Ee 287.15 ± 8.22Cd 3847.94 ± 132.12Ed
45 °C/ 4 weeks 1439.20 ± 31.50Cc 2407.34 ± 89.62BCbc 86.59 ± 0.36Cc 335.67 ± 8.30Bc 4268.80 ± 129.78CDc
45 °C/ 6 weeks 1685.79 ± 38.24Aa 2720.40 ± 84.92Aa 95.80 ± 0.34Aa 367.12 ± 8.14Aa 4869.12 ± 131.63Aa
  1. NNN N′-nitrosonornicotine; NAT N′-nitrosoanatabine; NAB N′-nitrosoanabasine; NNK 4-(methylnitrosamino)-1 -(3-pyridyl)-1-butanone; TSNAs Tobacco-specific Nitrosamines. Control group represents the sun-dried unfermented tobacco leaves. All data are represented by mean ± SD (n = 3). Uppercase letters indicate p-value < 0.05 and lowercase letters indicate p-value < 0.01