The stability of the coiled-coil structure near to N-terminus influence the heat resistance of harpin proteins from Xanthomonas

Table 1 Gene names, gene accession numbers and primers used in this study

Name	Accession number		Primer sequence (5′-3′)	PCR product size (bp)	Purpose
hpaXm	DQ643828.1	Forward	CGGGATCCATGAATTCTTTGAACACACAG (BamHI)^a	428	PCR for cloning
hpaXm	DQ643828.1	Reverse	AAGGAAAAAAGCGGCCGCTTACTGCATCGATCCGGTGTCGCT (NotI)^a	428	PCR for cloning
hpa1Xoo	CP000967.2	Forward	CGGGATCCATGAATTCTTTGAACACACAA (BamHI)^a	446	PCR for cloning
hpa1Xoo	CP000967.2	Reverse	AAGGAAAAAAGCGGCCGCTTACTGCATCGATGCGCTGTCGCT (NotI)^a	446	PCR for cloning
hpaXpm	KY765410.1	Forward	CGGGATCCATGAACCCAGCGGCGCAGACC (BamHI)^a	435	PCR for cloning
hpaXpm	KY765410.1	Reverse	CCGCTCGAGTTACTGCATCGATCCGGTGTCGCT (XhoI)^a	435	PCR for cloning
hpaXcm	KY697778.1	Forward	CGGGATCCATGATGAATTCTTTGAACACA (BamHI)^a	422	PCR for cloning
hpaXcm	KY697778.1	Reverse	CCGCTCGAGTTACTGCATCGATCCGGTGTCGCT (XhoI)^a	422	PCR for cloning

ISSN: 1471-2180