Fig. 2

Predictions of the PFC of Hpa1 and mutants, and detection their ability to induce HR. a Predictions of PFC in Hpa1 and mutants. Letters a - g designate the position of residues in the heptad repeats. The PFC was obtained by scanning windows of 14 residues using the MTIDK matrix. Amino acid residues that were replaced are shown in blue. The PFC value for each of the proteins is shown in red. b Western blot analysis of efficient expression of Hpa1, and their mutants. Wild-type Hpa1, their mutants and GST (negative control) were probed with a polyclonal antibody specific for GST and then a goat anti-rabbit lgG-HRP antibody. The induced bacterial cultures were ultra-sonicated followed by centrifugation. The resulting supernatants were used as soluble protein samples. M, molecular markers. Molecular mass marker in size (kDa) is indicated on the left-hand side. c The abilities of Hpa1 and mutants to induce HR. Tobacco leaves infiltrated with 10 μM Hpa1 or their mutant proteins. The size of the injection site is indicated by a solid black line