Fig. 5

AhR or Nrf2 inhibition prevents ILA-induced response in intestinal epithelial cells. mRNA expression in IEC treated with ILA alone or with AhR or Nrf2 antagonist. a CYP1A1 b GPX2 c NQO1 d SOD2 e IL-8 mRNA. Data is expressed as mean ± SEM with n = 6–8 of relative expression values fitted with a 3-paramenter dose response curve vs the log-transformed ILA concentration (Prism, v. 8.0, GraphPad Software, San Diego, CA). Statistical significance of the effect of each treatment on the means of relative expression of each target gene at the highest ILA concentration (10 mM) was determined using a one-way ANOVA analysis with a TukeyHSD posthoc comparison (R, version 3.5.2 (1)). For IL-8, the statistical differences between the means in the absence of ILA was as also determined by this method. Different letters represent significantly different means. A summary of statistics can be found in Supplemental Table 3