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Table 2 Effect of PLP on cysteine desulfurase activity of purified IscS2

From: Methanosarcina acetivorans contains a functional ISC system for iron-sulfur cluster biogenesis

Sample

Cysteine desulfurase activityb

IscS2

21.5 ± 1.2

IscS2 + PLP

33.8 ± 0.5

IscS2PLPa

35.1 ± 2.1

IscS2PLP + PLP

33.4 ± 0.5

  1. a IscS2 reconstituted with PLP
  2. b Cysteine desulfurase activity (nmol sulfur min− 1 mg− 1 IscS2) of 5 μM IscS2 or IscS2PLP in the absence or presence of additional PLP (50 μM). Results are means from triplicates ±1 standard deviation