Skip to main content

Table 2 Effect of PLP on cysteine desulfurase activity of purified IscS2

From: Methanosarcina acetivorans contains a functional ISC system for iron-sulfur cluster biogenesis

Sample Cysteine desulfurase activityb
IscS2 21.5 ± 1.2
IscS2 + PLP 33.8 ± 0.5
IscS2PLPa 35.1 ± 2.1
IscS2PLP + PLP 33.4 ± 0.5
  1. a IscS2 reconstituted with PLP
  2. b Cysteine desulfurase activity (nmol sulfur min− 1 mg− 1 IscS2) of 5 μM IscS2 or IscS2PLP in the absence or presence of additional PLP (50 μM). Results are means from triplicates ±1 standard deviation