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Fig. 1 | BMC Microbiology

Fig. 1

From: Methanosarcina acetivorans contains a functional ISC system for iron-sulfur cluster biogenesis

Fig. 1

Purified IscS2 binds PLP and is a homodimer. a SDS-PAGE analysis of purified IscS2 (cropped image of original). Lane 1, MW marker; lane 2, IscS2 (2.5 μg). b UV-visible spectra of 20 μM IscS2 (dotted line) or IscS2PLP (solid line) in 50 mM Tris pH 7.2, 150 mM NaCl. c Size-exclusion chromatography of IscS2. IscS2 (3.3 mg loaded) was analyzed by size-exclusion chromatography with 50 mM Tris pH 8.0, 150 mM NaCl, 2 mM DTT, 10% glycerol. The molecular weight of IscS2 was calculated from a standard curve (inset). The square represents the Ve/Vo of IscS2 with a calculated molecular weight of 84 kDa, consistent with homodimer (87 kDa)

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