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Table 2 The strains and plasmids used in this study

From: Rsp activates expression of the Cnt system in Staphylococcus aureus

Strains or plasmids Genotype or characteristic (s)a References
S. aureus strains
 USA300 JE2 Parental strain for the Nebraska Transposon Mutant Library [10]
 NE1304 rsp mutant from the Nebraska Transposon Mutant Library with erm gene insertion; Erm R [10]
 Newman fur::tet fur mutant with tet gene insertion, TetR [12]
 NCTC8325 zur::tet zur mutant with tet gene insertion, TetR [13]
 USA300 fur fur mutant with tet gene insertion, TetR This study
 USA300 zur zur mutant with tet gene insertion, TetR This study
 USA300 fur rsp fur rsp double mutant; TetR, ErmR This study
 USA300 zur rsp zur rsp double mutant; TetR, ErmR This study
E. coli strains
 SG13009 pREP4 (pQE-9-rsp) Derived from E. coli K12 harboring a plasmid to overexpressed rsp. [11]
Plasmids
 pZTN10 Escherichia coli/Staphylococcus aureus shuttle vector; AmpR, CmR [17]
 pTZN10-rsp (pRsp) pTZN10 derivative containing rsp gene from S. aureus; AmpR, CmR This study
  1. aAbbreviations: Erm R Erythromycin resistance, TetR Tetracycline resisatnce, AmpR Ampicillin resisatnce, CmR Chloramphenicol resistance