Table 2 The strains and plasmids used in this study

 USA300 JE2

Parental strain for the Nebraska Transposon Mutant Library

[10]

 NE1304

rsp mutant from the Nebraska Transposon Mutant Library with erm gene insertion; Erm ^R

[10]

 Newman fur::tet

fur mutant with tet gene insertion, Tet^R

[12]

 NCTC8325 zur::tet

zur mutant with tet gene insertion, Tet^R

[13]

 USA300 fur

fur mutant with tet gene insertion, Tet^R

This study

 USA300 zur

zur mutant with tet gene insertion, Tet^R

This study

 USA300 fur rsp

fur rsp double mutant; Tet^R, Erm^R

This study

 USA300 zur rsp

zur rsp double mutant; Tet^R, Erm^R

This study

 SG13009 pREP4 (pQE-9-rsp)

Derived from E. coli K12 harboring a plasmid to overexpressed rsp.

[11]

 pZTN10

Escherichia coli/Staphylococcus aureus shuttle vector; Amp^R, Cm^R

[17]

 pTZN10-rsp (pRsp)

pTZN10 derivative containing rsp gene from S. aureus; Amp^R, Cm^R

This study