Fig. 4From: Rsp activates expression of the Cnt system in Staphylococcus aureusGel mobility shift assay with purified Rsp protein mixed with the biotinylated 494-bp sequence upstream of cntK (cntk) and (cntA) 381-bp sequence upstream of cntA. Eight fmol of the biotin-labeled DNA was incubated with 1.5 μg of purified protein. The reaction mixture was incubated for 20 min at room temperature and analyzed by 5% nondenaturing polyacrylamide gel electrophoresis (PAGE). For the competition assay, a 200-fold excess of unlabeled specific DNA or nonspecific salmon sperm DNA was added to the reaction mixture prior to the incubationBack to article page