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Fig. 4 | BMC Microbiology

Fig. 4

From: Rsp activates expression of the Cnt system in Staphylococcus aureus

Fig. 4

Gel mobility shift assay with purified Rsp protein mixed with the biotinylated 494-bp sequence upstream of cntK (cntk) and (cntA) 381-bp sequence upstream of cntA. Eight fmol of the biotin-labeled DNA was incubated with 1.5 μg of purified protein. The reaction mixture was incubated for 20 min at room temperature and analyzed by 5% nondenaturing polyacrylamide gel electrophoresis (PAGE). For the competition assay, a 200-fold excess of unlabeled specific DNA or nonspecific salmon sperm DNA was added to the reaction mixture prior to the incubation

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