Fig. 1

Complementation of Rsp in rich media. Relative expression of cntK, cntA and cntE genes in the rsp mutant strain with an empty plasmid pTZN10 and a plasmid expression rsp (pRsp) relative to USA300 JE2 with the empty plasmid were assessed by RT-qPCR. The bars represent the means of relative expression from at least three independent experiments. The error bars show standard errors of the means. Statistical differences were determined by Mann-Whitney U Test. rsp mutant pTZN10 showed lower expression for the three genes (cntK, cntA, cntE) than the USA300 JE2 pTZN10 strain (*P < 0.05), having no differences between USA300 JE2 pTZN10 and the complementation strain rsp mutant pRsp