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Fig. 1 | BMC Microbiology

Fig. 1

From: Combination of modified carbapenem inactivation method (mCIM) and EDTA-CIM (eCIM) for phenotypic detection of carbapenemase-producing Enterobacteriaceae

Fig. 1

The procedure and interpretation of mCIM and eCIM. a. A 1-μL loopful of test CRE isolate is resuspended in two tubes containing 2 mL of TSB. One tube is devoid of EDTA (mCIM), while the other is supplemented with EDTA (eCIM). A meropenem (MEM) disk is submerged in each tube, and the tubes are incubated at 35 °C for 4 h ± 15 min. The disks are then removed from the tubes and placed on MH agar plates upon which a carbapenem-susceptible reporter E. coli ATCC 25922 has been freshly applied. The plates are incubated at 35 °C for 16 to 20 h before the zone sizes are recorded. b. Interpretation of mCIM and eCIM tests of 4 clinical K. pneumoniae isolates. Isolate 459 was carbapenemase negative; 429 had metallo-β-lactamase (blaNDM-5); 448 had serine carbapenemase (blaOXA-48); 451 had serine carbapenemase (blaKPC-2)

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BMC Microbiology

ISSN: 1471-2180

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