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Fig. 3 | BMC Microbiology

Fig. 3

From: Diversity of mucoid to non-mucoid switch among carbapenemase-producing Klebsiella pneumoniae

Fig. 3

Capsule quantification and visualization in M and NM variants. Uronic acid assay [25] was carried out to quantify capsular polysaccharides in the eight CPKp and their selected NM variants. In all but one couple (KP1), the NM variant exhibited a significant decrease in CPS production compared to the WT isolate. In particular, a stronger difference was noticed in the case of CNR128G5 (p-value ****), followed by CNR137J2 and KPA28006. India ink staining was performed to visually detect decrease/absence of capsule in the non-mucoid variant (right image). The scale bar in each image represent 5 μm. Non-mucoid isolates selected for each K. pneumoniae strain showed different mutation events (Table 2). M = mucoid, NM = non-mucoid

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