Fig. 5

MS2 analysis of supernatant from T. forsythia ΔampG, showing the presence of GlcNAc-anhMurNAc (a) and GlcNAc-MurNAc (b). The parent masses of m/z 479.22 [M + H]+ for GlcNAc-anhMurNAc and m/z 497.19 [M + H]+ for GlcNAc-MurNAc were in accordance with the theoretical masses of m/z 479.19 and m/z 497.20, respectively. The b1-ion (GlcNAc), the y1-ion (MurNAc/anhMurNAc), and the MurNAc signature peaks (denoted by a triangle) support the structural assignment