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Fig. 3 | BMC Microbiology

Fig. 3

From: Utilization of different MurNAc sources by the oral pathogen Tannerella forsythia and role of the inner membrane transporter AmpG

Fig. 3

Digestion products obtained upon incubation of E. coli PGN with cell extracts of T. forsythia, prepared from cultures grown with MurNAc or PGN. Samples were taken after several time points and analysed by LC-ESI-MS in negative ion mode, showing the presence of GlcNAc-MurNAc - m/z 495.18 [M-H]−, GlcNAc-anhMurNAc - m/z 477.17 [M-H]−, MurNAc - m/z 292.11 [M-H]− and anhMurNAc - m/z 274.09 [M-H]−. Measured masses were in accordance with the calculated masses of m/z 495.18, m/z 477.17, m/z 292.10, and m/z 274.09, respectively. Shown are the mean values of triplicates calculated from the area under the curve (AUC) (a). The disaccharide was confirmed to be GlcNAc-MurNAc based on the successful digestion with the N-acetylglucosaminidase NagZ, shown as the extracted ion chromatogram (EIC) upon elution from a SeQuant® ZIC®-pHILIC column, before (black) and after (red) enzyme digestion (b)

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BMC Microbiology

ISSN: 1471-2180

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