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Fig. 3 | BMC Microbiology

Fig. 3

From: PhoPQ two-component regulatory system plays a global regulatory role in antibiotic susceptibility, physiology, stress adaptation, and virulence in Stenotrophomonas maltophilia

Fig. 3

Roles of phoPQ TCS in physiological functions. a Roles of phoPQ TCS in bacterial growth. Overnight culture of bacterial cells was inoculated into fresh LB broth with an initial OD450nm of 0.15. The bacterial growth was monitored by recording the OD450nm every 3 h. Error bars represent the standard error of the mean. *, P < 0.01, significance calculated by Student’s t test. b Roles of phoPQ TCS in swimming motility. The logarithmic-phase bacterial culture was adjusted to OD450nm of 1.0. Five-microliter suspension was inoculated into the swimming agar (1% tryptone, 0.5% NaCl, and 0.15% agar) and the swimming zones were recorded after 48-h incubation at 37 °C. Bars represent the average values from three independent experiments. Error bars represent the standard error of the mean. *, P < 0.01, significance calculated by Student’s t test. c Roles of phoPQ TCS in secreted protease activity. The logarithmic-phase bacterial culture was adjusted to OD450nm of 1.0 and 40 μl bacterial aliquot were inoculated into the skim milk agar plate. The proteolytic activity of bacteria was assessed by measuring the transparent zones around the bacteria after incubation for 72 h at 37 °C. Bars represent the average values from three independent experiments. Error bars represent the standard error of the mean. *, P < 0.01, significance calculated by Student’s t test

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