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Fig. 2 | BMC Microbiology

Fig. 2

From: PhoPQ two-component regulatory system plays a global regulatory role in antibiotic susceptibility, physiology, stress adaptation, and virulence in Stenotrophomonas maltophilia

Fig. 2

Expression of PhoP transcript and PhoP protein in wild-type KJ, its derived mutants, and the complementary strains. a PhoP transcript levels. Overnight culture of bacterial cells was inoculated into fresh LB with an initial OD450nm of 0.15. Cells were grown aerobically for 5 h before measuring phoP transcript using qRT-PCR. Bars represent the average values from three independent experiments. Error bars represent the standard error of the mean. *, P < 0.01, significance calculated by Student’s t test. b PhoP protein levels. Whole bacterial cell lysates were denatured and separated by 14% SDS-PAGE and then transferred onto polyvinylidene difluoride (PVDF) membranes. The membrane was incubated with anti-PhoP antibodies and secondary mouse anti-rabbit IgG immunoglobulin conjugated to horseradish peroxidase. The reaction was developed with a mixture of diamino benzidine and 30% hydrogen peroxide. Full-length blots/gels are presented in Supplementary’s Figure S1

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