Fig. 7

Incubation with PUFAs alters biofilm formation in E. coli. Overnight cultures were pelleted, washed, resuspended in appropriate media and inoculated onto microtiter plates (starting OD ~ 0.1) in octuplet. Each culture was grown in the presence of 300 μM of the indicated fatty acids. After 24 h incubation, the biofilm assay by O’Toole was performed in complex (LB) and minimal media (CM9). The absorbance (OD₅₉₀) was measured using a Biotek Synergy microplate reader. Shown is a representative assay from two independent experiments. Each value represents the mean and standard deviation of 8 wells. Asterisks indicate p-values determined to be less than 0.001 when compared to the no fatty acid controls