Fig. 4
The effect of exogenous PUFAs on hydrophobic compound uptake in E. coli. a Bacteria were grown at 37 °C in CM9 with and without 300 μM of the indicated fatty acids to mid-log phase (OD = 0.8). Cultures were gently pelleted, washed with PBS and resuspended in an equal volume of PBS (OD600 = 0.6). The amount of CV in the supernatant following centrifugation was measured at regular intervals and expressed graphically as percentage of CV uptake. All standard deviations were less than 4% (not graphed for visual clarity). b Bacteria were grown at 37 °C in CM9 with and without 300 μM of the indicated fatty acids to mid-log phase (OD = 0.8). Cultures were gently pelleted, washed with PBS and resuspended in an equal volume of PBS (OD600 = 0.7). The amount of EtBr in the supernatant following centrifugation was measured as fluorescence emission intensity at 585 nm (excitation wavelength of 530 nm). Asterisks indicate significant difference (*,p < 0.002) as compared to control. c Bacteria were grown at 37 °C in CM9 with and without 300 μM of the indicated fatty acids to mid-log phase (OD = 0.8). Cultures were gently pelleted, washed with PBS and resuspended in an equal volume of PBS (OD600 = 0.4). Following addition of 20 μM EtBr, fluorescence intensity was measured every 5 m for 1 h (excitation wavelength of 545 nm; emission wavelength of 600 nm)