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Fig. 2 | BMC Microbiology

Fig. 2

From: Exogenous polyunsaturated fatty acids (PUFAs) promote changes in growth, phospholipid composition, membrane permeability and virulence phenotypes in Escherichia coli

Fig. 2

Thin-layer chromatography of phospholipids extracted from E. coli following growth with exogenous PUFAs. Bacteria were grown to exponential phase (OD ≈ 0.8) in CM9 media at 37 °C with or without 300 μM of the indicated fatty acids (linoleic acid [18:2], alpha-linoleic acid [18:3α], gamma-linolenic acid [18:3γ], dihomo-gamma-linolenic acid [20:3], arachidonic acid [20:4], eicosapentaenoic acid [20:5] and docosahexaenoic acid [22:6]) prior to Bligh and Dyer extraction of phospholipids and separation by TLC in the solvent system chloroform/methanol/acetic acid (65:25:10 v/v/v). The plate was charred and scanned to produce the final image

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BMC Microbiology

ISSN: 1471-2180

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